Journal: Advanced Science

Article Title: Development of Polymeric Nanoparticles for Blood–Brain Barrier Transfer—Strategies and Challenges

doi: 10.1002/advs.202003937

Figure Lengend Snippet: An overview of ligands used to target the BBB, their specific targets, and examples of animal models and cell lines used

Article Snippet: [ ] In addition, SynVivo commercialized a 3D microvascular network named SynBBB that has been employed to develop an in vitro neo‐natal BBB model. [ , ] Hesperos Inc., (Orlando, USA) also commercialized a BBB microfluidic chip tailored for culturing human‐derived iPS cells to investigate the transport mechanisms through the BBB.

Techniques: Glycoproteomics, Virus

Journal: Journal of Biomedical Optics

Article Title: Two-photon microscopy of cortical NADH fluorescence intensity changes: correcting contamination from the hemodynamic response

doi: 10.1117/1.3633339

Figure Lengend Snippet: Hematocrit and optical scattering coefficient of the whole blood as a function of the microvascular diameter.

Article Snippet: Here, we utilized realistic three-dimensional (3-D) microvascular anatomical networks of cortical microvasculature obtained by in vivo TPLSM imaging.

Techniques:

Journal: Journal of Biomedical Optics

Article Title: Two-photon microscopy of cortical NADH fluorescence intensity changes: correcting contamination from the hemodynamic response

doi: 10.1117/1.3633339

Figure Lengend Snippet: Comparison of the ray-tracing algorithm with the experimental data. (a) Maximum intensity projection (MIP) of a 400-μm-thick microvascular stack labeled with FITC in rat primary somatosensory cortex. (b) MIP of the 110-μm-thick reconstructed microvasculature obtained from (a) using microvascular graphing. Vessel diameters were presented with different shades of gray. (c, e) TPLSM images of NADH and SR101 fluorescence intensities at a depth of 110 μm. Scale bars are 100 μm. (d, f) Fluorescence intensity profiles (black dots) along the white dotted lines from (c) and (e), respectively. Solid lines represent the results of the full ray-tracing algorithms (fluorescence excitation and emission detection), while dashed lines represent ray-tracing results based on blood absorption of emission detection only.

Article Snippet: Here, we utilized realistic three-dimensional (3-D) microvascular anatomical networks of cortical microvasculature obtained by in vivo TPLSM imaging.

Techniques: Comparison, Labeling, Fluorescence

Journal: Journal of Biomedical Optics

Article Title: Two-photon microscopy of cortical NADH fluorescence intensity changes: correcting contamination from the hemodynamic response

doi: 10.1117/1.3633339

Figure Lengend Snippet: Dependence of detected relative NADH fluorescence intensity on microvascular structure and imaging parameters. (a–c) Relative NADH fluorescence intensity dependence on vessel diameter (5–80 μm) and lateral distance at imaging depths of 20, 50, and 100 μm, respectively. (d, e) summarize the information presented in (a–c). Contour lines represent 50 and 90% intensity levels. (f) Influence of objective NA on detected relative NADH signal in the presence of the pial vessel with 50-μm diameter at three imaging depths (20, 50, and 100 μm).

Article Snippet: Here, we utilized realistic three-dimensional (3-D) microvascular anatomical networks of cortical microvasculature obtained by in vivo TPLSM imaging.

Techniques: Fluorescence, Imaging

Journal: Journal of Biomedical Optics

Article Title: Two-photon microscopy of cortical NADH fluorescence intensity changes: correcting contamination from the hemodynamic response

doi: 10.1117/1.3633339

Figure Lengend Snippet: NADH fluorescence emission intensity change during respiratory arrest. (a) Maximum intensity projection of a 200-μm-thick microvascular stack labeled with FITC in rat SI cortex. (b) NADH fluorescence intensity map 100 μm below cortical surface. Scale bar: 100 μm. (c) Temporal profile of relative NADH fluorescence intensity changes from the ROI outlined by black line in (b). An initial 95 s of normal breathing (FiO2 = 21%) was followed by 70 s of respiratory arrest and subsequent return to normal breathing. The respiratory arrest period is marked by the black bar in (c).

Article Snippet: Here, we utilized realistic three-dimensional (3-D) microvascular anatomical networks of cortical microvasculature obtained by in vivo TPLSM imaging.

Techniques: Fluorescence, Labeling